Article-Genomic Labeling

CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci

We have combined CRISPR and dual-FRET MBs to develop the CRISPR/dual-FRET MB system that can be used to measure the dynamics of non-repetitive regions of distinct genomic loci in the human genome with as few as three unique sgRNAs.

Abstract:

Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genomic loci with only three unique sgRNAs.

See full-text: https://doi.org/10.1093/nar/gkz752

Yachen Ying 2019-08-31 PUBLICATION
CRISPR Dual-FRET MB Genomic labeling