publication – Article-HIV Assembly
In contrast to the general notion that vRNA only triggers Gag assembly and is dispensable for subsequent assembly, we found that vRNA is indispensable throughout assembly, scaffolding the formation of assembly intermediates and maintaining their architectures via balancing of external forces acting on the assembly environment. Read More ›
publication – Article-Genomic Labeling
We have developed a novel live-cell genomic imaging platform, termed CRISPR/MB, which comprises dCas9, an MB, and an sgRNA engineered with an MB target sequence in the stem–loop 2 region, and demonstrated its capacity for quantitative, dynamic and dual-color analysis of genomic loci in human cells. Read More ›
publication – Article-Autophagy
In this study, we demonstrated that targeting of misassembled viral complexes from the PM to lysosomes for degradation is driven by macroautophagy. The process involves p62, which potentially recognizes the endocytosed misassembled viral complexes as protein aggregates. Read More ›
publication – Article-Single-molecule RNA imaging
Using MBs that elicit a marginal level of nonspecific signals (2Me/PSLOOP MBs), we showed that single RNA transcripts engineered with as few as 8 tandem repeats of an MB target sequence can be detected at the single-molecule level with high accuracy under standard widefield fluorescence microscopy. Read More ›
publication – Article-Single-molecule RNA imaging
In this study, we systematically optimized the extent of PS modification of the 2Me backbone and successfully created a new molecular beacon platform that overcomes the shortcomings (false-positive signals) associated with conventional MBs. Read More ›