publication – Review-Molecular Beacon
In this review, emphasis is given to the progress made in advancing the MB technology toward live-cell single-molecule analysis. Read More ›
publication – Article-Genomic Labeling
We have combined CRISPR and dual-FRET MBs to develop the CRISPR/dual-FRET MB system that can be used to measure the dynamics of non-repetitive regions of distinct genomic loci in the human genome with as few as three unique sgRNAs. Read More ›
publication – Review-Genomic Labeling
In this review, we describe currently-available approaches for imaging single genomic loci in cells, with special focus on clustered regularly interspaced short palindromic repeats (CRISPR)-based imaging approaches. Read More ›
publication – Article-HIV Assembly
In contrast to the general notion that vRNA only triggers Gag assembly and is dispensable for subsequent assembly, we found that vRNA is indispensable throughout assembly, scaffolding the formation of assembly intermediates and maintaining their architectures via balancing of external forces acting on the assembly environment. Read More ›
publication – Article-Genomic Labeling
We have developed a novel live-cell genomic imaging platform, termed CRISPR/MB, which comprises dCas9, an MB, and an sgRNA engineered with an MB target sequence in the stem–loop 2 region, and demonstrated its capacity for quantitative, dynamic and dual-color analysis of genomic loci in human cells. Read More ›