publication – Article-RNA nanostructure

Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockade

CHENLAB Here, we develop self-assembled RNA nanostructures that can inhibit HIV-1 virus assembly, achieved through hybridization of multiple artificial small RNAs with a stem–loop structure (STL) that we identify as a prominent ligand of Gag that can inhibit virus particle production via STL-Gag interactions. Read More ›

publication – Article-Bimolecular Fluorescence Complementation

A Background Assessable and Correctable Bimolecular Fluorescence Complementation System for Nanoscopic Single-Molecule Imaging of Intracellular Protein–Protein Interactions

CHENLAB We have developed Background Assessable and Correctable-BiFC (BAC-BiFC) that enables physiologically relevant characterization of specific PPIs at the nanoscopic level not attainable by conventional BiFC. Read More ›

publication – Review-Molecular Beacon

Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging

CHENLAB In this review, emphasis is given to the progress made in advancing the MB technology toward live-cell single-molecule analysis. Read More ›

publication – Article-Genomic Labeling

CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci

CHENLAB We have combined CRISPR and dual-FRET MBs to develop the CRISPR/dual-FRET MB system that can be used to measure the dynamics of non-repetitive regions of distinct genomic loci in the human genome with as few as three unique sgRNAs. Read More ›

publication – Review-Genomic Labeling

Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms

In this review, we describe currently-available approaches for imaging single genomic loci in cells, with special focus on clustered regularly interspaced short palindromic repeats (CRISPR)-based imaging approaches. Read More ›